Improved enrichment broth for cultivation of fastidious organisms.

Plates are better than broth for recovery of fastidious organisms from some specimen material.

There has been considerable recent interest in the lack of value of broth cultures in diagnostic bacteriology, as noted by several publications in the Journal of Clinical Microbiology (2–4, 7). One important point that I believe has been demonstrated but not commented on is that, with some exceptions, agar cultures are better than broth cultures for the recovery of fastidious bacteria. This poi...

Progress in culture and subculture of spheroplasts and fastidious acid-fast bacilli isolated from intestinal tissues.

The efficiency of culture media was compared for the culture and subculture of very slowly growing acid-fast bacilli and spheroplast forms obtained from intestinal tissues of patients with Crohn's disease and ulcerative colitis and from controls without inflammatory bowel disease. Media were developed by modifying a nutrient broth medium based on veal infusion broth and yeast extract. We evalua...

Evaluation of BacT/ALERT system for detection of Mycoplasma hominis in simulated blood cultures.

We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical isolates of M. hominis was maintained for 7 days in BacT/ALERT media, and org...

The use of Preston enrichment broth for the isolation of 'thermophilic' campylobacters from water.

The application of Preston enrichment broth to isolation of 'thermophilic' campylobacters from water has been investigated. Enrichment substantially increased the yield of such organisms. The optimum timing for subculturing the broth was after 48 h incubation. Despite the addition of aerotolerant supplement to the broth the number and variety of isolates was greater when the broth was incubated...

Swine Erysipelas Combination Assay for Rapid Diagnosis of Use of an Enrichment Broth Cultivation-PCR